Archaebacterial class I and class II aldolases from extreme halophiles

Details Archaebacterial class I and class II aldolases from extreme halophiles Archaebacterial class I and class II aldolases from extreme halophiles

Mar 1988

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1988orli...18...59a&link_type=abstract

Origins of Life and Evolution of the Biosphere, Volume 18, Issue 1-2, pp. 59-64

Physics

Scientific paper

Both, class I (Schiff-base forming) and class II (metal requiring) fructose biphosphate aldolases were found to be distributed among halophilic archaebacteria. The aldolase activity fromHalobacterium halobium, H. salinarium, H. cutirubrum, H. mediterranei andH. volcanii exhibited properties of a bacterial class II aldolase as it was metal-dependent for activity and therefore inhibited by EDTA. In contrast, aldolase fromH. saccharovorum, Halobacterium R-113, H. vallismortis andHalobacterium CH-1 formed a Schiff-base intermediate with the substrate and therefore resembled to eukaryotic class I type. The type of aldolase did not vary by changes in the growth medium.

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