An immunohistochemical technique for the localization of preserved biopolymeric remains in fossils

Physics

Scientific paper

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Scientific paper

Antibodies raised against the EDTA-soluble organic matrix from Recent shells of the bivalve mollusk Mercenaria mercenaria , proved to be specific for heterodont and palaeoheterodont bivalves, were used to demonstrate the in situ localization of biopolymers in shells of Recent and Pleistocene (75,000 year old) Mercenaria . The bipolymer antigens were detected by immunohistochemical staining and visualized by light microscopy. The organic matrix surrounding the prisms in the Recent shell was heavily stained, whereas fewer antigens were located within the constituent crystallites. A similar pattern was seen in the Pleistocene sample, but the staining was less precise, indicating some diagenetic alteration of the matrix antigens. The in situ localization of antigenic determinants amplifies the repertoire of techniques available for the study of original organic remains in the fossil record.

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