Biology – Quantitative Biology – Biomolecules
Scientific paper
2004-08-18
Biology
Quantitative Biology
Biomolecules
14 pages, 2 figures
Scientific paper
10.1063/1.1937990
Recently, it has been confirmed that long duplex DNA molecules with sizes larger than several tens of kilo-base pairs (kbp), exhibit a discrete conformational transition from an elongated coil state to a compact globule state upon the addition of various kinds of chemical species that usually induce DNA condensation. In this study, we performed a single-molecule observation on a large DNA, Lambda ZAP II DNA (ca. 41 kbp), in a solution containing RNA polymerase and substrates along with spermine, a tetravalent cation, at different concentrations, by use of fluorescence staining of both DNA and RNA. We found that transcription, or RNA production, is completely inhibited in the compact state, but is actively performed in the unfolded coil state. Such an all-or-none effect on transcriptional activity induced by the discrete conformational transition of single DNA molecules is discussed in relation to the mechanism of the regulation of large-scale genetic activity.
Kubo Koji
Nakai Tonau
Tsumoto Kanta
Yamada Ayako
Yoshikawa Kenichi
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