Adaptive optics in wide-field microscopy

Details Adaptive optics in wide-field microscopy Adaptive optics in wide-field microscopy

Feb 2011

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2011spie.7931e..18k&link_type=abstract

MEMS Adaptive Optics V. Edited by Olivier, Scot S.; Bifano, Thomas G.; Kubby, Joel A. Proceedings of the SPIE, Volume 7931, p

Physics

Optics

Scientific paper

Three-dimensional live imaging in cell biology is hindered by optical aberrations which degrade the resolution and signal-to-noise ratio as the focal plane is moved deeper into the sample. The solution to this problem is to use adaptive optics to correct the aberrations. In this paper, we discuss our work on applying adaptive optics to wide-field fluorescence microscopy. We demonstrate correction of depth-aberrations and focusing using a deformable mirror in open-loop operation. We then discuss the use of phase retrieval and phase diversity in adaptive optics.

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