Actin network architecture and elasticity in lamellipodia of melanoma cells

Details Actin network architecture and elasticity in lamellipodia of melanoma cells Actin network architecture and elasticity in lamellipodia of melanoma cells

Nov 2007

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2007njph....9..420f&link_type=abstract

New Journal of Physics, Volume 9, Issue 11, pp. 420 (2007).

Physics

1

Scientific paper

Cell migration is an essential element in the immune response on the one hand and in cancer metastasis on the other hand. The architecture of the actin network in lamellipodia determines the elasticity of the leading edge and contributes to the regulation of migration. We have implemented a new method for the analysis of actin network morphology in the lamellipodia of B16F1 mouse melanoma cells. This method is based on fitting multi-layer geometrical models to electron microscopy images of lamellipodial actin networks. The chosen model and F-actin concentrations are thereby deterministic parameters. Using this approach, we identified distinct structural features of actin networks in lamellipodia. The mesh size which defines the elasticity of the lamellipodium was determined as 34 and 78 nm for a two-layer network at a total actin concentration of 9.6 mg ml-1. These data lead to estimates of the low frequency elastic shear moduli which differ by more than a magnitude between the two layers. These findings indicate an anisotropic shear modulus of the lamellipodium with the stiffer layer being the dominant structure against deformations in the lamellipodial plane and the softer layer contributing significantly at lower indentations perpendicular to the lamellipodial plane. This combination creates a material that is optimal for pushing forward as well as squeezing through narrow spaces.

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