Physics – Optics
Scientific paper
2009-07-29
Review of Scientific Instruments 80, 8 (2009) 083702
Physics
Optics
Accepted for publication in Review of Scientific Instrument
Scientific paper
10.1063/1.3205447
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes, are reported.
Aillaud A.
Ferrand P.
Kress Achim
Marguet D.
Pianta M.
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