Physics – Biological Physics
Scientific paper
2001-10-28
Nuclear Instruments & Methods in Physics Research, vol. A 470 (2001) p. 409-416
Physics
Biological Physics
PDF: 10 pages, 6 figures. MS Word sourse
Scientific paper
10.1016/S0168-9002(01)01087-7
The small-angle X-ray and neutron scattering, time resolved X-ray small-angle and wide-angle diffraction coupled with differential scanning calorimetry have been applied to the investigation of unilamellar and multilamellar dimyristoylphosphatidylcholine (DMPC) vesicles in sucrose buffers with sucrose concentrations from 0 to 60%. Sucrose buffer decreased vesicle size and polydispersity and increased an X-ray contrast between phospholipid membrane and bulk solvent sufficiently. No influence of sucrose on the membrane thickness or mutual packing of hydrocarbon chains has been detected. The region of sucrose concentrations 30%-40% created the best experimental conditions for X-ray small-angle experiments with phospholipid vesicles.
Killany M.
Kiselev M. A.
Kisselev A. M.
Lesieur P.
Lesieur S.
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