A Non-dialysable, Heat-stabile, Hypertensive Substance in the Serum of Rats

Details A Non-dialysable, Heat-stabile, Hypertensive Substance in the Serum of Rats A Non-dialysable, Heat-stabile, Hypertensive Substance in the Serum of Rats

Nov 1964

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1964natur.204..581k&link_type=abstract

Nature, Volume 204, Issue 4958, pp. 581-582 (1964).

Physics

1

Scientific paper

INCUBATION of rat serum at 37° C, without any digestion by proteolytic enzymes or change of the pH, effects the liberation of a highly pressure-active substance. This hypertensive effect appears after an incubation of 5 h and reaches its maximum after 15 h at 37° C (Fig. 1A). The blood pressure of rats nephrectomized 24-48 h before or after application of a ganglionic blocking agent or infusion of hypotonic glucose is controlled. 0.25 ml. of incubated serum given intravenously causes a rise of the blood pressure of about 35 mm mercury; this equals, in terms of pressor activity, 0.05 (µg of `Hypertensin' (CIBA). Infusion of a hypertonic sodium solution diminishes the hypertensive effect both of the incubated serum and of synthetic angiotensin (hypertensin (CIBA)). Incubation of serum during a period longer than 40 h slowly reduces the hypertensive activity of incubated serum. The hypertensive substance is not dialysable against sodium chloride. Ultrafiltration across a cellulose membrane is not possible (Fig. 1B). The pressure-active principle is found at 40-60 per cent saturation with ammonium sulphate. Boiling of this fraction at pH 1 for 10 min is sustained without any reduction in hypertensive effect; at pH 12 the hypertensive principle is destroyed.

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