A Light Source for locating Œstrogens on Chromatograms

Physics

Scientific paper

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Scientific paper

As part of an investigation at this Station into the reproductive physiology of sheep, a suitable light source was required for detecting traces of œstrogens on paper chromatograms of blood extracts. (Œstrogens have an absorption maximum near 280 mµ, and light near this wave-length gives maximum contrast when used for photographing them on filter paper. The mercury-vapour lamp is commonly used for this purpose, but its emission in this region is weak and a train of filters is required.

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