A Factor associated with `Visking' Tubing capable of Antagonizing Insulin

Physics

Scientific paper

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Scientific paper

DURING the investigation of the insulin antagonism associated with human serum albumin1, `Visking' tubing (Visking Corporation, Chicago, U.S.A,) was used for ultrafiltration and dialysis of protein solutions. The tubing itself has since been found to give rise to a substance which antagonizes insulin and which appears in the following circumstances: (1) When distilled water is ultrafiltered through 7-ft. of heavy grade `Visking' tubing (27/32 in. inflated diameter) supported by `Terylene' net, under a negative pressure of 40-50 cm. mercury, over a period of three days. (2) When 7 ft. light grade `Visking' tubing (18/32 in.) is filled with water and suspended in changes of distilled water for 2-7 days, that is, used as dialysis tubing.

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