Physics – Medical Physics
Scientific paper
Jan 2000
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2000aipc..504..582f&link_type=abstract
SPACE TECHNOLOGY AND APPLICATIONS INTERNATIONAL FORUM - 2000. AIP Conference Proceedings, Volume 504, pp. 582-587 (2000).
Physics
Medical Physics
1
Growth And Division, Aerospace Bio- And Medical Physics, Spaceborne And Space Research Instruments, Apparatus, And Components
Scientific paper
The requirement for long term storage of cell cultures previous to arrival on the International Space Station (ISS), as well as culture maintenance after the conduct of experiment in microgravity, necessitates inhibition of cell proliferation and metabolism pending return to earth-based laboratories. Transport of cells in a nonstabilized condition can lead to a loss of cell viability and/or a source of selection pressures for survival that can alter the overall cell population. We have isolated in our laboratory a reversible inhibitor of cell proliferation, a cell regulatory sialoglycopeptide (CeReS-18), that has the capability of stabilizing cells isolated from a wide phylogenetic range by arresting them in the G1 phase of the cell cycle. We show here that CeReS-18 is unusually stable and can be stored at ambient temperatures for weeks without a measurable loss in its biological activity. In addition we demonstrate that CeReS-18 is a superior cell-stabilizing agent as compared to other methods deployed for cell stabilization purposes, such as, decrease in the incubation temperature and serum down shifts. We also discovered that hybridoma cultures stabilized in their proliferative cycle by CeReS-18 produced 150%-300% more antibody per cell than that measured in the proliferating control cultures. The reversible inhibitory activity of CeReS-18, together with its unusual stability, as well as its wide target range lend themselves to use of this inhibitor as a cell stabilizing agent for cell transport to and storage on the ISS. .
Consigli Richard A.
Fattaey Heideh K.
Grenz Ladonna
Johnson Terry C.
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