13 C / 12 C Fractionation of methane during oxidation in a temperate forested soil

Physics

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Scientific paper

We have made measurements of the 13 C / 12 C fractionation of methane (CH 4 ) during microbial oxidation by an upland temperate soil from College Woods, New Hampshire, using both in situ and laboratory incubation measurements. Uptake rates of 1-4.8 mg CH 4 /m 2 /d were measured during the active season in New Hampshire while rates of uptake were 2.6-6.8 mg CH 4 /m 2 /d in jars used for incubation studies. The fractionation factor, calculated from field measurements, was = 0.978 ± .004. This corresponds to a kinetic isotope effect (KIE) of ki 2 / k 13 = 1.022 ± .004. Only a small dependence on temperature was noted for air temperatures between 281 and 296 K. Our results indicate that the KIE of soil CH 4 oxidation is controlled by physical parameters based on gaseous diffusion into the soil. The implications of these results are discussed with respect to the global CH 4 budget and balancing CH 4 sources and sinks through the use of 13 CH 4 measurements.

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