Biology – Quantitative Biology – Biomolecules
Scientific paper
2006-12-05
Europ J Medic Res., 2006, Oct. 8, vol.11 - p. 128-129
Biology
Quantitative Biology
Biomolecules
On the base of thesis of 17th European students' Conference, 8-12 October 2006, Berlin, Germany
Scientific paper
Protein splicing is a post-translational autocatalystic excision of internal protein sequence (intein) with the subsequent ligation of the flanking polypeptides (exteins). The high specificity of excision ensured by intein makes it possible to use a phenomenon of protein splicing for the biotechnology purposes. The aim of this work was optimization of obtaining and purification of the recombinant human growth hormone using the protein splicing. It was experimentally demonstrated that the use of modified intein as auto-removal affine marker makes it possible to perform the rapid and cheap isolation of the recombinant protein Hgh. Furthermore, this approach allows to obtain the human growth hormone with native N-terminus, without formyl-metionine. Key words: intein, human growth hormone, protein splicing
Dubey L. V.
Okunev Oleg V.
Starokadomskyy P. L.
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