Recoiling DNA Molecule: Simulation & Experiment

Details Recoiling DNA Molecule: Simulation & Experiment Recoiling DNA Molecule: Simulation & Experiment

2002-03-27

arxiv.org/abs/physics/0203087v2

Physica A 345, 173-184 (2005)

Physics

Biological Physics

14 pages (single column preprint), 7 figures. Major changes: data analysis method improved; dna-ethidium bromide results remov

Scientific paper

Single molecule DNA experiments often generate data from force versus extension measurements involving the tethering of a microsphere to one end of a single DNA molecule while the other is attached to a substrate. We show that the persistence length of single DNA molecules can also be measured based on the recoil dynamics of these DNA-microsphere complexes if appropriate corrections are made to the friction coefficient of the microsphere in the vicinity of the substrate. Comparison between computer simulated recoil curves, generated from the corresponding Langevin equation, and experimental recoils is used to assure the validity of data analysis.

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