Orientation of the Mitochondria during Mitosis

Physics

Scientific paper

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Scientific paper

LITTLE is known concerning the behaviour of the mitochondria during cell division. Therefore we have studied the mitosis of cells from the tail of the pancreas in very young albino mice of the Danish State Serum Institute strain. The mice were three to eight days old (weight, 3-3½ gm.). For vital staining of the pancreatic cells the technique of Scott1 was used. The carotids were clamped, a needle, wire gauge 26-27 (outer diameter, 0.5 mm.; inner diameter, 0.22 mm.), was introduced into the left ventricle of the heart, and Janus Green B solution 1/100,000 was injected at a pressure of 250-300 mm. mercury in the course of some fifteen minutes. The heart was contracting during the injection.

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