In situ Detection of β -Galactosidase in Lenses of Transgenic Mice with a γ -Crystallin/lacZ Gene

Details In situ Detection of β -Galactosidase in Lenses of Transgenic Mice with a γ -Crystallin/lacZ Gene In situ Detection of β -Galactosidase in Lenses of Transgenic Mice with a γ -Crystallin/lacZ Gene

Jan 1987

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1987sci...235..456g&link_type=abstract

Science, Volume 235, Issue 4787, pp. 456-458

Physics

3

Scientific paper

Transgenic mice carrying the γ 2-crystallin promoter fused to the coding region of the bacterial lacZ gene were generated. The offspring of three founder mice expressed high levels of the enzyme solely in the central nuclear fiber cells of the lens as measured by an in situ assay for the detection of β -galactosidase activity. These results suggest that γ 2-crystallin sequences between -759 to +45 contain essential information required for appropriate tissue-specific and temporal regulation of the mouse γ 2-crystallin gene. In a broader context, this study also demonstrates the utility of β -galactosidase hybrid gene constructs for monitoring the activity of gene regulatory elements in transgenic mice.

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