Urease reaction rates at low water activity

Details Urease reaction rates at low water activity Urease reaction rates at low water activity

Aug 1971

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1971slsci...3....3s&link_type=abstract

Space Life Sciences, Volume 3, Issue 1, pp.3-11

Physics

Scientific paper

Hydrolysis of urea by urease takes place in ‘dry’ urea-urease mixture exposed to discrete water vapor pressures from 100% to 20% relative humidity and at 2°C to 70°C. A discontinuity in enzymatic activities is observed at the transition of urea from a solid to a deliquescent solution. Urease is inactivated more readily at higher relative humidities in saturated urea solution than at lower relative humidities where water for urea hydrolysis is adsorbed on enzyme-protein only. Hydrolysis of urea by urease proceeds at a measurable rate in concentrated solutions of urea and of urea hydrolysis products, ammonium carbonate and bicarbonate, in the absence of ionic strength or pH stabilizing agents.

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