Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability

Details Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability

Nov 2006

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2006njph....8..275b&link_type=abstract

New Journal of Physics, Volume 8, Issue 11, pp. 275 (2006).

Physics

1

Scientific paper

We demonstrate the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by photoswitching ensembles of optically bistable organic molecular markers from a non-fluorescent to a fluorescent state and back. The photoswitching is accomplished by an isomerization reaction of a photochromic compound serving as a reversible energy acceptor of a fluorescent compound. The surpassing of the diffraction barrier with power levels of only a few hundred W cm-2 of continuous wave irradiation is evidenced both in the effective point spread function and in the fluorescence images of test samples.

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