Vesicles containing ion channels on crystalline surfaces-An FTIR and surface enhanced FTIR spectroscopic study

Details Vesicles containing ion channels on crystalline surfaces-An FTIR and surface enhanced FTIR spectroscopic study Vesicles containing ion channels on crystalline surfaces-An FTIR and surface enhanced FTIR spectroscopic study

Jun 1998

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1998aipc..430..594f&link_type=abstract

The eleventh international conference on fourier transform spectroscopy. AIP Conference Proceedings, Volume 430, pp. 594-597 (1

Physics

Infrared And Raman Spectroscopy

Scientific paper

The kinetics of the adsorption of native vesicles containing the nicotinic acetylcholine receptor (nAChR) is monitored by ATR-FTIR and SEIRA spectroscopy. The membrane vesicles are adsorbed on Ge crystals. Experiments are done with neat Ge and with Ge crystals covered with a thin layer of silver clusters in order to obtain the enhancement effect of infrared adsorption. The nAChR shows β-sheet/turn structures at the interface. These results give evidence for the existence of these structures in the extracellular domains of the receptor. The potential for SEIRA in the investigation of proteins at interfaces and membrane processes is outlined.

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