Influence of buoyancy-driven convection on protein separation by free-flow electrophoresis

Details Influence of buoyancy-driven convection on protein separation by free-flow electrophoresis Influence of buoyancy-driven convection on protein separation by free-flow electrophoresis

Jul 1992

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1992adspr..12..373c&link_type=abstract

Advances in Space Research, Volume 12, Issue 1, p. 373-383.

Physics

Scientific paper

In free-flow electrophoresis, the stability and reproducibility of the flow field are prerequisites for a satisfactory separation. Due to the presence of the species to be separated and due to mass- and heat-transfer phenomena, non-uniformities in density inevitably appear, both within the carrier buffer and between the carrier buffer and the sample stream. On earth they give rise to buoyancy-driven convection which interferes with the separation. These effects have been quantified by numerical modelling. Agreement has been found between the numerical results and experimental observations.

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