Heterogeneity of Titan Yellow demonstrated by Thin-layer Chromatography

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Scientific paper

SINCE the first observations by Mikkelsen and Toth1 it has been well established that the reaction between magnesium ions and titan yellow (the sodium salt of methylbenzothiazole (1,3)-4,4'-diazoamino benzene (2,2')-disulphonic acid) can be extremely variable due to differences in the composition of the dye. In an attempt to overcome difficulties in the determination of magnesium in plant samples due to such variations Bradfield2 recommended a technique for assessing the sensitivity of the dye and later reported the presence of a component which was intensely fluorescent3.

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