Biology – Quantitative Biology – Tissues and Organs
Scientific paper
2011-03-20
PLoS ONE 6(6): e21303 (2011)
Biology
Quantitative Biology
Tissues and Organs
* The first two authors contributed equally to this work
Scientific paper
10.1371/journal.pone.0021303
To understand dynamic developmental processes, living tissues must be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in indeterminately growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image at cellular resolution a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, track cellular nuclei and identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics.
Birnbaum Kenneth D.
Frentz Zak
Leibler Stanislas
Sena Giovanni
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