Two-dimensional gel electrophoresis in proteomics: A tutorial

Details Two-dimensional gel electrophoresis in proteomics: A tutorial Two-dimensional gel electrophoresis in proteomics: A tutorial

2011-09-14

arxiv.org/abs/1109.3178v1

Journal of Proteomics 74, 10 (2011) 1829-41

Biology

Quantitative Biology

Genomics

Scientific paper

10.1016/j.jprot.2011.05.040

Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the process, from sample preparation to in-gel detection of proteins, commenting the constraints and caveats of the technique. Then the limitations and positive features of two-dimensional electrophoresis are discussed (e.g. its unique ability to separate complete proteins and its easy interfacing with immunoblotting techniques), so that the optimal type of applications of this technique in current and future proteomics can be perceived. This is illustrated by a detailed example taken from the literature and commented in detail. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 2).

Affiliated with

Also associated with

No associations

Rating

Two-dimensional gel electrophoresis in proteomics: A tutorial does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Two-dimensional gel electrophoresis in proteomics: A tutorial, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Two-dimensional gel electrophoresis in proteomics: A tutorial will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-570494