Progress in the definition of a reference human mitochondrial proteome

Details Progress in the definition of a reference human mitochondrial proteome Progress in the definition of a reference human mitochondrial proteome

2006-11-24

arxiv.org/abs/q-bio/0611075v1

Proteomics 3 (02/2003) 157-67

Biology

Quantitative Biology

Genomics

website publisher http://www.interscience.wiley.com

Scientific paper

10.1002/pmic.200390024

Owing to the complexity of higher eukaryotic cells, a complete proteome is likely to be very difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized tools to study specifically the biology and "physiology" of the target organelle. Within this frame, we report here the construction of the human mitochondrial proteome, using placenta as the source tissue. Protein identification was carried out mainly by peptide mass fingerprinting. The optimization steps in two-dimensional electrophoresis needed for proteome research are discussed. However, the relative paucity of data concerning mitochondrial proteins is still the major limiting factor in building the corresponding proteome, which should be a useful tool for researchers working on human mitochondria and their deficiencies.

Affiliated with

Also associated with

No associations

Rating

Progress in the definition of a reference human mitochondrial proteome does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Progress in the definition of a reference human mitochondrial proteome, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Progress in the definition of a reference human mitochondrial proteome will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-540432