Biology – Quantitative Biology – Genomics
Scientific paper
2006-04-12
Biology
Quantitative Biology
Genomics
22 Pages, 7 Figures, 1 Table
Scientific paper
10.1016/j.physa.2006.06.004
Affymetrix Genechip microarrays are used widely to determine the simultaneous expression of genes in a given biological paradigm. Probes on the Genechip array are atomic entities which by definition are randomly distributed across the array and in turn govern the gene expression. In the present study, we make several interesting observations. We show that there is considerable correlation between the probe intensities across the array which defy the independence assumption. While the mechanism behind such correlations is unclear, we show that scaling behavior and the profiles of perfect match (PM) as well as mismatch (MM) probes are similar and immune to background subtraction. We believe that the observed correlations are possibly an outcome of inherent non-stationarities or patchiness in the array devoid of biological significance. This is demonstrated by inspecting their scaling behavior and profiles of the PM and MM probe intensities obtained from publicly available Genechip arrays from three eukaryotic genomes, namely: Drosophila Melanogaster, Homo Sapiens and Mus musculus across distinct biological paradigms and across laboratories, with and without background subtraction. The fluctuation functions were estimated using detrended fluctuation analysis (DFA) with fourth order polynomial detrending. The results presented in this study provide new insights into correlation signatures of PM and MM probe intensities and suggests the choice of DFA as a tool for qualitative assessment of Affymetrix Genechip microarrays prior to their analysis. A more detailed investigation is necessary in order to understand the source of these correlations.
Nagarajan Radhakrishnan
Upreti Meenakshi
No associations
LandOfFree
Qualitative Assessment of Gene Expression in Affymetrix Genechip Arrays does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Qualitative Assessment of Gene Expression in Affymetrix Genechip Arrays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Qualitative Assessment of Gene Expression in Affymetrix Genechip Arrays will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-515166