Silver Staining of Proteins in 2DE Gels

Details Silver Staining of Proteins in 2DE Gels Silver Staining of Proteins in 2DE Gels

2009-04-22

arxiv.org/abs/0904.3535v1

Methods in molecular biology (Clifton, N.J.) 519 (2009) 339-350

Biology

Quantitative Biology

Genomics

Scientific paper

10.1007/978-1-59745-281-6_21

Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 day after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.

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