Three-dimensional microscopy using a diffraction grating primary objective

Computer Science

Scientific paper

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Scientific paper

Three-dimensional magnification of a diffusing surface can be achieved using profilometry based on holographic chirped frequency gratings and laser line projection. The recently patented method being investigated both supplants earlier 3-D microscopy methods using diffraction range finders and also competes favorably with extant confocal microscopy in coverage area, speed of acquisition and unit cost. The geometry of grazing incidence in a diffraction range finder shows an intrinsic anamorphic magnification. A narrow waist of input rays is expanded to the full width of the grating itself. The spatial magnification is simply the ratio of the length of the grating to the waist of the input column of rays. As the column of input rays approaches the grating plane, this magnification ratio approaches infinity. We develop a theory that predicts a limit to resolution as a function of illumination wave length and grating pitch of the primary objective. A simple microscope can achieve near micron resolution over several centimeters of depth in the visible light regime. An empirical demonstration is made using silver halide holograms.

No associations

LandOfFree

Say what you really think

Search LandOfFree.com for scientists and scientific papers. Rate them and share your experience with other people.

Rating

Three-dimensional microscopy using a diffraction grating primary objective does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Three-dimensional microscopy using a diffraction grating primary objective, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Three-dimensional microscopy using a diffraction grating primary objective will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFWR-SCP-O-1473232

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.