Detection of mercury compounds using invertase-glucose oxidase-based biosensor

Details Detection of mercury compounds using invertase-glucose oxidase-based biosensor Detection of mercury compounds using invertase-glucose oxidase-based biosensor

Oct 1995

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1995spie.2504..209a&link_type=abstract

Proc. SPIE Vol. 2504, p. 209-220, Environmental Monitoring and Hazardous Waste Site Remediation, Tuan Vo-Dinh; Ed.

Computer Science

Scientific paper

Mercury compounds have been determined with an electrochemical biosensor based on invertase inhibition. When invertase is in the presence of mercury its activity decreases; this causes a decrease of glucose production which is monitored by the glucose sensor and correlated to the concentration of mercury in solution. Parameters as pH, enzyme concentration, substrate concentration, and reaction and incubation time were optimized. Mercury compounds determination using soluble or immobilized invertase were reported. Results show that the inhibition was competitive and reversible. Mercury compounds can be detected directly in aqueous solution in the range 2 - 10 ppb.

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