What can we learn from noncoding regions of similarity between genomes?

Biology – Quantitative Biology – Genomics

Scientific paper

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10 pages, 5 figures

Scientific paper

Background: In addition to known protein-coding genes, large amount of apparently non-coding sequence are conserved between the human and mouse genomes. It seems reasonable to assume that these conserved regions are more likely to contain functional elements than less-conserved portions of the genome. Here we used a motif-oriented machine learning method to extract the strongest signal from a set of non-coding conserved sequences. Results: We successfully fitted models to reflect the non-coding sequences, and showed that the results were quite consistent for repeated training runs. Using the learned model to scan genomic sequence, we found that it often made predictions close to the start of annotated genes. We compared this method with other published promoter-prediction systems, and show that the set of promoters which are detected by this method seems to be substantially similar to that detected by existing methods. Conclusions: The results presented here indicate that the promoter signal is the strongest single motif-based signal in the non-coding functional fraction of the genome. They also lend support to the belief that there exists a substantial subset of promoter regions which share common features and are detectable by a variety of computational methods.

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