Mathematics – Logic
Scientific paper
Oct 2006
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2006jap...100g4701f&link_type=abstract
Journal of Applied Physics, Volume 100, Issue 7, pp. 074701-074701-8 (2006).
Mathematics
Logic
3
Biological Techniques And Instrumentation, Biomedical Engineering, Cellular Structure And Processes
Scientific paper
Intracytoplasmic sperm injection (ICSI) is regarded as a critical assisted reproductive technology procedure in which a single spermatozoon is mechanically injected into the cytoplasm of an oocyte using an injection pipette. During the ICSI procedure, a spermatozoon is loaded into the injection pipette and the pipette is then inserted into the oocyte cytoplasm (ooplasm). Insertion of the injection pipette in such way to minimize damage to the oocyte is facilitated by a piezoactuator connected to the pipette. Importantly, to minimize lysis of the oocyte during ICSI, the injection pipettes driven by the piezoactuator are typically filled with approximately 0.5-1.0 μL of mercury. The use of mercury in the pipettes is associated with a significantly lower frequency of oocyte lysis, theoretically due to the reduced lateral vibration of the pipette when the mass of mercury is added. However, the use of mercury in the laboratory has serious potential toxicity effects, both related to personnel safety and to the developmental competence of the manipulated oocytes. Therefore, the current study was developed to analyze the effects of mercury on the vibration characteristics of the piezodriven pipette, with the long-term goal of developing an improved method which does not require the use of mercury. In this study, the injection pipette is modeled as a cantilever beam immersed in a viscous liquid. The forces on the pipette by the surrounding liquid include both the inertial force and the viscous force. The steady state response of the pipette is obtained by finite element method together with numerical integration methods. We investigate the pipette dynamic responses when different fluids are used as the plug inside the pipette and as the fluid surrounding the pipette. Based on the analysis, we propose that the effect of mercury on the vibration is not the key reason that facilitates the ICSI.
Agca Yuksel
Critser John K.
Fan Mingxuan
Feng Z. C.
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