Two-dimensional gel electrophoresis in proteomics: past, present and future

Details Two-dimensional gel electrophoresis in proteomics: past, present and future Two-dimensional gel electrophoresis in proteomics: past, present and future

2010-08-16

arxiv.org/abs/1008.2591v1

Journal of proteomics (2010) epub ahead of print

Biology

Quantitative Biology

Genomics

Scientific paper

10.1016/j.jprot.2010.05.016

Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist. The events that have led to the birth of proteomics are also recalled, ending with a description of the now well-known limitations of two-dimensional gels in proteomics. However, the often-underestimated advantages of two-dimensional gels are also underlined, leading to a description of how and when to use two-dimensional gels for the best in a proteomics approach. Taking support of these advantages (robustness, resolution, and ability to separate entire, intact proteins), possible future applications of this technique in proteomics are also mentioned.

Affiliated with

Also associated with

No associations

Rating

Two-dimensional gel electrophoresis in proteomics: past, present and future does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Two-dimensional gel electrophoresis in proteomics: past, present and future, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Two-dimensional gel electrophoresis in proteomics: past, present and future will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-177014