Transport of S-Antigen of Sendai Virus as revealed by means of Antibody labelled with an Organic Mercury Compound

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IT has been shown that replication of RNA1 and synthesis of S-antigen of Sendai virus occurs in nucleolar structures while synthesis of V-antigen occurs in the perinuclear zone of cytoplasm2. In later stages of infection both components, ribonucleoprotein (RNA + S-antigen) and haemagglutinin (V-antigen), accumulate in the peripheral zone of cytoplasm where mature virions are formed. A modification of the immunofluorescence method3 has revealed the details of synthesis of protein components of Sendai virus, but failed to reveal the details of transport of S-antigen through the territory of nucleus and cytoplasm because it was insufficiently sensitive. Therefore a more sensitive method of detection of S-antigen of Sendai virus was used, namely, antibody labelled with an organic mercury compound, para-aminophenylmercurylacetate (pAPMA)4.

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