Tracking of individual freely diffusing fluorescent protein molecules in the bacterial cytoplasm

Details Tracking of individual freely diffusing fluorescent protein molecules in the bacterial cytoplasm Tracking of individual freely diffusing fluorescent protein molecules in the bacterial cytoplasm

2010-03-10

arxiv.org/abs/1003.2110v1

Biology

Quantitative Biology

Quantitative Methods

Scientific paper

We combine stroboscopic laser excitation with stochastic photoactivation and super-resolution fluorescence imaging. This makes it possible to record hundreds of diffusion trajectories of small protein molecules in single bacterial cells with millisecond time resolution and sub-diffraction limited spatial precision. We conclude that the small protein mEos2 exhibits normal diffusion in the bacterial cytoplasm with a diffusion coefficient of 13.1 -+ 1.2 \mu m^2 s^(-1). This investigation lays the groundwork for studying single-molecule binding and dissociation events for a wide range of intracellular processes.

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