The Light Microscopy Module Design and Performance Demonstrations

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Scientific paper

The Light Microscopy Module (LMM) is planned as a remotely controllable, automated, on-orbit microscope subrack facility, allowing flexible scheduling and control of fluids and biology experiments within the Fluids and Combustion Facility (FCF) Fluids Integrated Rack (FIR) on the International Space Station (ISS). The LMM recently completed the preliminary design phase, and is progressing toward engineering model development. This paper will provide a description of the LMM preliminary design, LMM planned capabilities and key features, a summary of demonstrations for each of the diagnostics, and future plans. In addition, a brief description of the initial four experiments will be provided. Key diagnostic capabilities for meeting science requirements include video microscopy to observe microscopic phenonema and dynamic interactions, interferometry to make thin film measurements with nanometer resolution, laser tweezers for particle manipulation, confocal microscopy to provide enhanced three-dimensional visualization of structures, and spectrophotometry to measure photonic properties of materials. The LMM also provides experiment sample containment for frangibles and fluids. Sample cell design allows investigators to perform experiments and observations on a variety of sample materials, including potential biological specimens. The LMM features high resolution video microscopy, brightfield, darkfield, phase contrast, differential interference contrast (DIC), and spectrophotometry in the visible 400 nm to 700 nm range. Laser tweezers is a custom-built system, shown to be capable of trapping up to a 5x5 array of colloidal particles. The LMM confocal system includes a commercial-off-the-shelf unit which utilizes a 532 nm frequency doubled Nd:Yag laser. This instrument has been demonstrated to provide images up to 80 microns deep in collodial samples. Spectrophotometry is a custom-built system that utilizes existing optical components within the microscope in addition to a commercial monochrometer for wavelength discrimination, a mechanism for varying the illumination angle relative to the sample, and the FIR-provided metal halide white light source. Tests have been conducted on the spectral response of the light source, microscope objective, and the monochrometer. Results of these tests indicates the spectral response is within the visible range of the microscope. In addition to individual diagnostic tests, the LMM will be subjected to environmental testing to investigate the response of the instument within an expected ISS environment, with a priority on vibration testing to clarify LMM operating constraints.

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