The interactions between penetration enhancers and human skin assessed by FT-Raman spectroscopy

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Infrared And Raman Spectroscopy, Other Fiber-Optical Devices

Scientific paper

FT-Raman spectra have been recorded from human stratum corneum in vivo and in vitro using a fibre optic coupling. Spectra from both tissues were near identical illustrating the validity of in vitro studies for examining the mechanisms of action of penetration enhancers in vivo. Treatment of excised human skin with the penetration enhancer 1,8-cineole showed that the permeation promoter induced increased order in the barrier bilayered lipids domains of the stratum corneum in most of the samples, but caused a decrease in order of these domains in some of the samples investigated. This may correlate with a mechanism of action where the enhancer exists as a discrete domain within the stratum corneum lipids which would provide defects in the barrier nature of the permeation limiting lipid domains.

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