Computer Science
Scientific paper
Aug 1999
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1999oleb...29..375j&link_type=abstract
Origins of Life and Evolution of the Biosphere, v. 29, Issue 4, p. 375-390 (1999).
Computer Science
2
Scientific paper
The first living systems may have employed template-directed oligonucleotide ligation for replication. The utility of oligonucleotide ligation as a mechanism for the origin and evolution of life is in part dependent on its fidelity. We have devised a method for evaluating ligation fidelity in which ligation substrates are selected from random sequence libraries. The fidelities of chemical and enzymatic ligation are compared under a variety of conditions. While reaction conditions can be found that promote high fidelity copying, departure from these conditions leads to error-prone copying. In particular, ligation reactions with shorter oligonucleotide substrates are less efficient but more faithful. These results support a model for origins in which there was selective pressure for template-directed oligonucleotide ligation to be gradually supplanted by mononucleotide polymerization.
Ellington Andrew D.
James Kenneth D.
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