Computer Science
Scientific paper
Jan 2001
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2001adspr..28..555t&link_type=abstract
Advances in Space Research, Volume 28, Issue 4, p. 555-561.
Computer Science
Scientific paper
We examined whether microgravity influences the induced-mutation frequencies through in vivo experiments during space flight aboard the space shuttle Discovery (STS-91). We prepared dried samples of repair-deficient strains and parental strains of Escherichia (E.) coli and Saccharomyces (S.) cerevisiae given DNA damage treatment. After culture in space, we measured the induced-mutation frequencies and SOS-responses under microgravity. The experimental findings indicate that almost the same induced-mutation frequencies and SOS-responses of space samples were observed in both strains compared with the ground control samples. It is suggested that microgravity might not influence induced-mutation frequencies and SOS-responses at the stages of DNA replication and/or DNA repair. In addition, we developed a new experimental apparatus for space experiments to culture and freeze stocks of E. coli and S. cerevisiae cells.
Amano Takanobu
Masukawa M.
Nagaoka Satoshi
Nakano Takanori
Ohnishi Katsuhiko
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