Computer Science – Other Computer Science
Scientific paper
2007-11-21
Dans Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS - DTIP 2006, Stresa, Lago Maggiore : Italie (2006)
Computer Science
Other Computer Science
Submitted on behalf of TIMA Editions (http://irevues.inist.fr/tima-editions)
Scientific paper
Thermalcycler were extensively used machine for amplify DNA sample. One of the major problems in the working time was that it spent most of time for cooling and heating. In order to improve the efficient, this study presented a novel method for amplify DNA sample. For this concept, the DNA sample in the silicon chamber which was pushed by a beam through three temperature regions around a center and then the DNA segments could be amplified rapidly after 30 cycles. The polymerase chain reaction platform was composed of thin-film heaters, copper plates, DC powers, and temperature controllers. The photolithography and bulk etching technologies were utilized to construct the thin-film heater and DNA reaction chambers. Finally, 1 pound gL 100bp DNA segment of E. coli K12 was amplified successfully within 36 minutes on this PCR platform.
Chien Ch.-Heng
Yu Hui-Min
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