Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination

Details Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination

2008-11-19

arxiv.org/abs/0811.3163v1

Proc. Natl. Acad. Sci. USA 105, 18159-18164 (2008)

Biology

Quantitative Biology

Subcellular Processes

includes Supporting Information

Scientific paper

10.1073/pnas.0806084105

In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic model, integrating results of single-molecule and quantitative in vivo studies of E. coli, to evaluate the quantitative effect of pausing, termination, and antitermination on rRNA transcription. Our calculations reveal that in dense RNAP traffic, spontaneous pausing of RNAP can lead to severe "traffic jams", as manifested in the broad distribution of inter-RNAP distances and can be a major factor limiting transcription and hence growth. Our results suggest the suppression of these pauses by the ribosomal antitermination complex to be essential at fast growth. Moreover, unsuppressed pausing by even a few non-antiterminated RNAPs can already reduce transcription drastically under dense traffic. However, the termination factor Rho can remove the non-antiterminated RNAPs and restore fast transcription. The results thus suggest an intriguing role by Rho to enhance rather than attenuate rRNA transcription.

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