Step-scan versus rapid-scan FTIR-VCD spectroscopy using HgCdTe and InSb detectors

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Infrared Spectrometers, Auxiliary Equipment, And Techniques, Photodetectors

Scientific paper

A comparison of step-scan and rapid-scan Fourier transform vibrational circular dichroism (FT-VCD) spectroscopy in the hydrogen-stretching region has been carried out on Nicolet Magna 850 and Bruker IFS-55 FTIR spectrometers, each equipped with an accessory VCD bench. Single enantiomer VCD spectra of (+)- and (-)-camphor with flat baselines are obtained on each instrument, both in step-scan mode and rapid-scan mode. On both instruments, the RMS noise level is less than 1×10-6 (absorbance units) in less than two-hour collections, and the noise level obtained with the step-scan method is slightly lower than that for rapid-scan. The calibrated noise level of VCD spectra obtained on the Nicolet instrument is about twice that obtained on the Bruker instrument for a comparable collection time. Our preliminary results show that with an InSb detector on the Nicolet instrument, the noise level of VCD spectra is about a half of that obtained with an MCT detector.

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