Size- and geometry-dependent trapping efficiency of the optical stretcher

Details Size- and geometry-dependent trapping efficiency of the optical stretcher Size- and geometry-dependent trapping efficiency of the optical stretcher

Apr 2002

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2002aps..apr.x4002s&link_type=abstract

American Physical Society, April Meeting, Jointly Sponsored with the High Energy Astrophysics Division (HEAD) of the American As

Astronomy and Astrophysics

Astrophysics

Scientific paper

The optical stretcher is a novel two-beam optical trap that has been shown to be capable of stretching biological cells and other soft dielectrics [1]. Rather than using focused laser light, as in a conventional optical trap, the stretcher employs two counter propagating diverging beams emitted by single-mode optical fibers that are separated by a few hundred microns. The resulting net radiation force on a symmetrical object centered in the stretcher is zero, but the surface force resulting from momentum conservation as the photon enters and exits the cell are sufficient to produce large stresses. The stretcher is being used to study the mechanical properties of living cells at greater stresses and with less photodamage than with other techniques. We have modeled the stretcher using a computational wave optics approach that naturally incorporates diffraction effects. With this model, we have evaluated the stretching force and the efficiency of the stretcher for trapping objects of arbitrary size and geometry for a variety of stretcher configurations. Preliminary findings reveal that the stability of the trap is highly sensitive to the size of the trapped object as expected. Results obtained for several object geometries and stretcher configurations will be discussed. [1] J. Guck et al., Biophysical J. 81: 767-784, 2001.

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