Residual chromatin breaks as biodosimetry for cell killing by carbon ions

Details Residual chromatin breaks as biodosimetry for cell killing by carbon ions Residual chromatin breaks as biodosimetry for cell killing by carbon ions

Nov 1998

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1998adspr..22.1663s&link_type=abstract

Advances in Space Research, Volume 22, Issue 12, p. 1663-1671.

Other

Scientific paper

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET = 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour post-irradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.

Affiliated with

Also associated with

No associations

Rating

Residual chromatin breaks as biodosimetry for cell killing by carbon ions does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.

If you have personal experience with Residual chromatin breaks as biodosimetry for cell killing by carbon ions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Residual chromatin breaks as biodosimetry for cell killing by carbon ions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFWR-SCP-O-1074078