Mathematics – Logic
Scientific paper
Mar 2011
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=2011opten..50c4402y&link_type=abstract
Optical Engineering, Volume 50, Issue 3, pp. 034402-034402-6 (2011).
Mathematics
Logic
Biosensors, Gold, Lab-On-A-Chip, Surface Plasmon Resonance, Biosensors, Biological Techniques And Instrumentation, Biomedical Engineering, Collective Excitations
Scientific paper
Surface plasmon resonance is particularly important due to the ability of biomolecules of interest to interact with a specific binding partner, and may therefore be more informative than generic measurement techniques. A growing number of robust and reproducible immobilized surfaces chips are available from Biacore, allowing us to analyze various biomolecular interactions. Here we describe a protocol by which the Biacore chips may be reused multiple times to analyze biomolecular interactions without interfering analysis from previously used surfaces or analytes. This procedure will not only help to extend the lifetime of these chips but at the same time render them to be more commercially affordable, especially in a resource-poor setting. The time range for the entire protocol is ~1 day, including stripping off previously immobilized materials and re-functionalization of gold surface.
Gopinath Subash C. B.
Kumar Penmetcha K. R.
Yuan Yong J.
No associations
LandOfFree
Regeneration of commercial Biacore chips to analyze biomolecular interactions does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Regeneration of commercial Biacore chips to analyze biomolecular interactions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Regeneration of commercial Biacore chips to analyze biomolecular interactions will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1402132