Radiocarbon dating of peptides isolated from contaminated fossil bone collagen by collagenase digestion and reversed-phase chromatography

Details Radiocarbon dating of peptides isolated from contaminated fossil bone collagen by collagenase digestion and reversed-phase chromatography Radiocarbon dating of peptides isolated from contaminated fossil bone collagen by collagenase digestion and reversed-phase chromatography

Jun 1994

adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1994gecoa..58.2543v&link_type=abstract

Geochimica et Cosmochimica Acta, vol. 58, Issue 11, pp.2543-2551

Mathematics

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Scientific paper

The peptide mixture, resulting from collagenase (enzymatic) cleavage of fossil bone collagen, is separated into a fraction containing the very bone-specific tripeptides glycine-proline-hydroxyproline (GlyProHyp), and glycine-proline-alanine (GlyProAla) and more hydrophobic fractions, using reversedphase chromatography. The suitability of these fractions for 14 C bone dating and stable isotope analysis is discussed, firstly by assessing the theoretical benefits of their use as compared to other bone-specific extracts, and secondly by examining 14 C ages of the fractions in a selection of bones that had previously been giving dubious results using the Oxford routine bone pretreatment. In most cases, the age obtained on the tripeptides GlyProHyp and GlyProAla was in better agreement with the expected age than the original 14 C measurement. In some cases the ages of the fractions were similar, indicating more of an archaeological than a radiocarbon dating problem. We conclude that the use of GlyProHyp/GlyProAla is the best solution to date for 14 C dating of contaminated bone samples.

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