Biology – Quantitative Biology – Quantitative Methods
Scientific paper
2003-09-30
Journal of Biomedical Optics Vo.8, 362 (July 2003)
Biology
Quantitative Biology
Quantitative Methods
Overview of FLIM techniques, StreakFLIM instrument, FRET applications
Scientific paper
10.1117/1.1577574
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent developments in multiphoton FLIM methods and also present a description of a novel multiphoton FLIM system using a streak camera that was developed in our laboratory. We provide an example of a typical application of the system in which we measure the fluorescence resonance energy transfer between a donor/acceptor pair of fluorescent proteins within a cellular specimen.
Centoze V. E.
Herman Benjamin
Krishnan R. V.
Masuda Akimasa
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