Biology – Quantitative Biology – Quantitative Methods
Scientific paper
2006-01-09
Biophys. J. 90, L17-L19 (2006)
Biology
Quantitative Biology
Quantitative Methods
3 pages (Letter); 1 figure
Scientific paper
10.1529/biophysj.105.075176
Single-molecule tracking is a powerful way to look at the dynamic organization of plasma membranes. However, there are some limitations to its use. For example, it was recently observed, using numerical simulation, that time-averaging effects inherent to the exposure time of detectors are likely to bias the apparent motion of molecules confined in microdomains. Here, we solve this apparently limiting issue analytically. We explore this phenomenon by calculating its effects on the observed diffusion coefficients and domain sizes. We demonstrate that the real parameters can be easily recovered from the measured apparent ones. Interestingly, we find that single-molecule tracking can be used to explore events occurring at a timescale smaller than the exposure time.
Destainville Nicolas
Salome Laurence
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