Biology – Quantitative Biology – Quantitative Methods
Scientific paper
2009-07-31
Biology
Quantitative Biology
Quantitative Methods
16 pages, 3 figures, revised
Scientific paper
Standard deviation measurements of intensity profiles of stationary single fluorescent molecules are useful for studying axial localization, molecular orientation, and a fluorescence imaging system's spatial resolution. Here we report on the analysis of the precision of standard deviation measurements of intensity profiles of single fluorescent molecules imaged using an EMCCD camera. We have developed an analytical expression for the standard deviation measurement error of a single image which is a function of the total number of detected photons, the background photon noise, and the camera pixel size. The theoretical results agree well with the experimental, simulation, and numerical integration results. Using this expression, we show that single-molecule standard deviation measurements offer nanometer precision for a large range of experimental parameters.
DeCenzo Shawn H.
DeSantis Michael C.
Li Je-Luen
Wang Yu-Ming
No associations
LandOfFree
Precision analysis for standard deviation measurements of single fluorescent molecule images does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Precision analysis for standard deviation measurements of single fluorescent molecule images, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Precision analysis for standard deviation measurements of single fluorescent molecule images will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-154538