Mathematics – Logic
Scientific paper
Jul 1992
adsabs.harvard.edu/cgi-bin/nph-data_query?bibcode=1992oleb...21..225b&link_type=abstract
Origins of Life and Evolution of the Biosphere, Volume 21, Issue 4, pp.225-242
Mathematics
Logic
1
Scientific paper
Some properties of DNA condensed with spermidine have been compared with the properties of DNA condensed with Co3+(NH3)6 to determine whether condensation of DNA with these trivalent cations protects DNA against the action of DNase I and increases transcription and encapsulation of DNA into liposomes. It was shown that DNA condensed with Co3+(NH3)6 was resistant to the action of the endonuclease DNase I such as DNA condensed with spermidine was. However, DNA condensed with Co3+(NH3)6 was significantly less active in transcription with theE. coli RNA polymerase than DNA-spermidine condensed forms. In addition, it was demonstrated that both compacted forms of DNA were more efficiently encapsulated into neutral liposomes; however, negatively, charged liposomes were scarcely formed in the presence of DNA condensed with Co3+(NH3)6. These experiments and the well documented properties of polyamines increasing the resistance to radiations and hydrolysis of nucleic acids, as well as their biological activities, such as replication, transcription, and translation, together with the low concentration of Co3+ in the environment, lead us to propose spermidine as a plausible prebiotic DNA condensing agent rather than Co3+ and the basic proteins proposed by other authors. Then, we consider the possible role and relevance of the polyamine-nucleic acids complexes in the evolution of life.
Baeza Isabel
Chávez Pedro
Gariglio Patricio
Ibanez Miguel
Oro Judit
No associations
LandOfFree
Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA does not yet have a rating. At this time, there are no reviews or comments for this scientific paper.
If you have personal experience with Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Possible prebiotic significance of polyamines in the condensation, protection, encapsulation, and biological properties of DNA will most certainly appreciate the feedback.
Profile ID: LFWR-SCP-O-1210799