Pixel multiplexing for high-speed multi-resolution fluorescence imaging

Details Pixel multiplexing for high-speed multi-resolution fluorescence imaging Pixel multiplexing for high-speed multi-resolution fluorescence imaging

2009-10-05

arxiv.org/abs/0910.0789v1

Biology

Quantitative Biology

Quantitative Methods

3 pages, 2 figures, submitted to Nature Methods

Scientific paper

We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imaging calcium transients in heart cells at 250 Hz with a 10 Hz megapixel camera.

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