Organic matter within crystalline aggregates of hydroxyapatite: A new substrate for stable isotopic and possibly other biogeochemical analyses of bone

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Scientific paper

The portion of organic matrix in bones that is present within fused aggregates of hydroxyapatite crystals was isolated by oxidizing the rest of the bone organic matter with sodium hypochlorite. The aggregate organic matter from a variety of modern and prehistoric bones was subjected to elemental and stable C and N isotopic analysis. For comparison, collagen from modern bones and the fraction from prehistoric bones with the same solubility characteristics as collagen (referred to herein as "collagen") were subjected to the same analytical procedures. Collagen and aggregate organic matter in modern bones have similar 15 N values but dissimilar 13 C values. The difference may be caused by the presence of non-collagenous proteins (NCPs) in the aggregate organic matter, because the NCPs have 13 C values different from those of collagen from the same bone. The organic matter in aggregates is not subject to the same diagenetic processes that can alter the isotope ratios of collagen, and appears to retain an in vivo isotope signal even in cases in which that of "collagen" has been altered. These conclusions apply to samples that were burned prehistorically as well as to those that suffered postmortem alteration only in the depositional environment. The organic matter in aggregates represents a new substrate that should prove useful for stable isotopic studies and possibly for radiocarbon and other biogeochemical analyses of bone.

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